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. 2020 Mar 3;21(5):1718. doi: 10.3390/ijms21051718

Figure 5.

Figure 5

PP1 regulates repression of RAB20 by Ikaros. (A) Human B-ALL cell lines were treated with pharmacological PP1 inhibitors calyculin and tautomycin. qChIP was used to determine Ikaros occupancy at the RAB20 promoter. (B) RAB20 expression was analyzed using qRT-PCR after PP1 inhibition with calyculin and tautomycin. (C) HEK293T cells were transduced with wild-type Ikaros (IKZF1(WT)), Ikaros with the mutated PP1 binding-site (IKZF1(565-7A)), and Ikaros with the PP1 binding-site mutated and phosphoresistant alanine mutations at CK2 phosphosites (IKZF1(465-7A + A11)). qChIP was used to determine Ikaros binding at the RAB20 promoter. (D) qRT-PCR was used to determine RAB20 expression in HEK293T cells transduced with wild-type Ikaros (IKZF1-(WT)) and mutated Ikaros (IKZF1-(465-7A) and IKZF1-(465-7A + A11)). The graphed data are the mean ± SD (error bars) from three independent experiments.