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. 2020 Mar 10;21(5):1901. doi: 10.3390/ijms21051901

Figure 5.

Figure 5

Effects of hyaluronic acid (HA) and decreased concentrations of dexamethasone (Dex) on CSE-impaired primary human chondrocytes. Cells (N ≥ 3, n ≥ 3) were incubated with Dex (4, 40, and 400 μg/mL) or HA (0.1, 1, and 5 mg/mL) with or without 10% CSE. After 7 days of treatment, viability of the cells was measured by (a, e) mitochondrial activity (resazurin conversion) and (b, f) total protein content (SRB staining). (c, g) Matrix formation of cells was evaluated by quantifying Alcian blue staining. (d) AP activity of chondrocytes in the presence of different concentrations of Dex on day 7 and day 14. Results are given as fold change of control (untreated cells, represented in red line or red dotted line) and presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. the control group, ° p < 0.05, °° p < 0.01, °°°° p < 0.0001compared to cells treated with CSE alone.