Table 1.
Primer name for target gene and the expression control genes (housekeeping), and forward or reverse sequence are indicated. The optimal annealing temperature was 55 °C in all cases. CaPCS2 = phytochelatin synthase gene. All oligonucleotide primers used in the study were previously reported [19].
| PCR Primer | Gene | Sequence (5′ to 3′) | F/R |
|---|---|---|---|
| CaPCH2_3F | CaPCS2 (target gene) | TGGGATTGGGATATTGTGCT | F |
| CaPCH2_2R | ATCTGTTTATGCCCCTGCAC | R | |
| 18SrRNAF | 18S rRNA (housekeeping) | TCAACTTTCGATGGTAGGATAGTG | F |
| 18SrRNAR | CCGTGTCAGGATTGGGTAATTT | R | |
| RT46_ACT1 | Actin (housekeeping) | CTCACTCTCAACATTCCAGCAA | F |
| RT46_ACT2 | GGGCCCGCTCTCATCATACTC | R |
PCR—polymerase chain reaction; F—forward sequence; R—reverse sequence.