Figure 3.

Pro-regenerative activity of IL-33 in the cell cultures exposed to H. pylori components. (A) Migration effectiveness of the guinea pig primary gastric epithelial cells (i) or fibroblasts (ii), not transfected or transfected with siRNA IL-33, cultured in medium only or in the milieu of H. pylori glycine acid extract (GE), expressed as the percentage of wound confluence in the scratch assay. (B) Proliferation of the guinea pig primary gastric epithelial cells (i) or fibroblasts (ii) untransfected or transfected with siRNA IL-33 in medium only or in the milieu of H. pylori GE, estimated in a radioactive assay. Proliferation is shown as stimulation index (SI), which was calculated, expressed as cpm ratio of unstimulated vs GE stimulated cells. (C) Metabolic activity of the guinea pig primary gastric epithelial cells (i) or fibroblasts (ii), not transfected or transfected with siRNA IL-33, expressed as the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction by stimulated cells relative to unstimulated cells. Results are showed as a median with a range of five experiments performed in triplicates for each experimental variant. Statistical analysis was performed using the nonparametric U Mann-Whitney test with significance for *, ** p < 0.05, * unstimulated (only medium) vs. GE stimulated, untransfected or tranfected cells, ** untransfected vs. transfected cells in medium only or in medium with GE.