Figure 6.

The expression of anti-apoptotic Bcl-xL and cleaved caspase 3 in cell cultures in vitro. Levels of anti-apoptotic Bcl-xL and cleaved caspase 3 (CC3) in cell cultures of guinea pig primary gastric epithelial cells (A) or fibroblasts (B), untransfected or transfected with siRNA cultured in medium only or exposed to H. pylori glycine acid extract (GE ).The level of intensity of Bcl-xL (i) and CC3 (iii) was measured using the Image J software version 1.48v (National Institute of health, United States). Representative images of cells stained for Bcl-xL (ii) or CC3 (iv) and photographed under 63x magnification in confocal microscope (LeicaTCS SP, Wetzlar, Germany)). 4′,6-diamino-2-phenylindole (DAPI) was used for nuclear staining and phalloidin –Texas Red^TM for cytoskeleton staining. The results of four independent experiments performed in triplicates for each experimental variant are presented. Statistical analysis was performed using the nonparametric U Mann-Whitney test with significance for *, ** p < 0.05, * unstimulated (only medium) vs. GE stimulated, untransfected or tranfected cells, ** untransfected vs. transfected cells in medium only or in medium with GE.