Figure 4.

OsGW2 T-DNA insertion knock-out mutant (gw2-ko) in wild-type Dongjin genetic background. (a) Schematic diagram showing the location of T-DNA insertion of the PFG_1B-10017.R mutant. (b) T-DNA insertion was confirmed using two sets of primers indicated in (a), and lines homozygous for the T-DNA insertion were used for this experiment. (c) Comparison of grain shape between wild type and gw2-ko. (d) Confirmation of OsGW2 knock-out by semi-quantitative RT-PCR. OsUBQ5 was used as a loading control. (e) Gene expression pattern of OsGW2 during the dark-induced senescence. The transcript level of genes was determined by qRT-PCR. OsUBQ5 was used for normalization.