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. 2020 Feb 25;21(5):1552. doi: 10.3390/ijms21051552

Figure 4.

Figure 4

Effects of sterols on the dynamics of Flot1-GFP spots at the plasma membrane. (A) Distribution of Flot1-GFP motion range in control (n = 9987 spots) and in cpi1-1 mutant (n = 5316 spots) seedlings. (B) Frequency of long-distance and short-distance motions for Flot1-GFP without flg22 treatment. Error bars represent the SD (n = 15–20). * p < 0.05, Student’s t-test. (C) Distribution of Flot1-GFP diffusion coefficients in control (n = 10389 spots) and in cpi1-1 mutant (n = 5316 spots) seedlings. (D) Diffusion coefficients of Flot1-GFP without flg22 treatment. Error bars represent the SD (n = 15–20). * p < 0.05, Student’s t-test. (E) Distribution of Flot1-GFP motion range in control (n = 8896 spots) and in cpi1-1 mutant (n = 7737 spots) seedlings after flg22 treatment (15 min). (F) Frequency of long-distance and short-distance motions of Flot1-GFP after flg22 treatment. Error bars represent the SD (n = 15–20). * p < 0.05, Student’s t-test. (G) Distribution of Flot1-GFP diffusion coefficients in control (n = 8896 spots) and in cpi1-1 mutant (n = 7737 spots) seedlings after flg22 treatment (15 min). (H) Diffusion coefficients of Flot1-GFP after flg22 treatment. Error bars represent the SD (n = 15–20). * p < 0.05, Student’s t-test.