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. 2020 Feb 27;21(5):1624. doi: 10.3390/ijms21051624

Figure 6.

Figure 6

Validation of gga-miR-221-5p and the ELOVL6 and SQLE targeting relationship. (A,B) Recombinant plasmid sequencing. (C,D) Dual luciferase reporter assay analysis of the target relationship of gga-miR-221-5p with ELOVL6 and SQLE in LMH chicken hepatoma cells. The long box □ indicates the position of the gga-miR-221-5p seed region in combination with the ELOVL6 and the SQLE gene 3′UTR, the black vertical line indicates the position of the ELOVL6 and SQLE gene 3′UTR deletion binding to the gga-miR-221-5p seed region. WT indicates wild, Mut indicates mutation, + indicates added, - indicates not added. Data are represented as mean ± SD (n = 6). ** means 0.001 < p < 0.01, *** means p < 0.0001.