Figure 8.

Effect of Tmspz4 gene silencing on expression of NF-κB genes. Three different pathogens, E. coli, S. aureus, and C. albicans, were injected into TmSpz4-silenced T. molitor young larvae, and the expression of the NF-κB genes TmDorX1 (A), TmDorX2 (B), and TmRel (C) were then investigated by RT-qPCR. Larvae were injected with dsEGFP as a negative control, and TmL27a expression was assessed as an internal control. All experiments were performed in triplicate. Asterisks indicate significant differences in NF-κB gene expression between the dsTmSpz4- and dsEGFP-treated groups when compared by Student’s t-test (p < 0.05).