Table 1.
Sequences of the primers used in this study.
| Primer Name | Sequence (5′-3′) |
|---|---|
| TmSpz4-qPCR-Fw | GGCGATGCTCTTCCAGGAC |
| TmSpz4-qPCR-Rv | CGCGTTCACTCCTTTCATTTGG |
| TmSpz4-T7-Fw | TAATACGACTCACTATAGGGTCCAGATGTACTGTCGCGATG |
| TmSpz4-T7-Rv | TAATACGACTCACTATAGGGTTTCCTTCTGTACCAGTCGGG |
| TmSpz4-cloning-Fw | ACCGACACAACCACCAAAAG |
| TmSpz4-cloning-Rv | ATCCGTGATCTCCGGAAAAA |
| TmSpz4-cloning-FullORF-Fw | GACGGTGCCCACGAACTAT |
| TmSpz4-cloning-FullORF-Rv | AAGAGCACGAGCCTTGACAT |
| TmL27a_qPCR_Fw | TCATCCTGAAGGCAAAGCTCCAGT |
| TmL27a_qPCR_Rv | AGGTTGGTTAGGCAGGCACCTTTA |
| dsEGFP_Fw | TAATACGACTCACTATAGGGTCGTAAACGGCCACAAGTTC |
| dsEGFP_Rv | TAATACGACTCACTATAGGGT TGCTCAGGTAGTGTTGTCG |
| TmTenecin-1_Fw | CAGCTGAAGAAATCGAACAAGG |
| TmTenecin-1_Rv | CAGACCCTCTTTCCGTTACAGT |
| TmTenecin-2_Fw | CAGCAAAACGGAGGATGGTC |
| TmTenecin-2_Rv | CGTTGAAATCGTGATCTTGTCC |
| TmTenecin-3_Fw | GATTTGCTTGATTCTGGTGGTC |
| TmTenecin-3_Rv | CTGATGGCCTCCTAAATGTCC |
| TmTenecin-4_Fw | GGACATTGAAGATCCAGGAAAG |
| TmTenecin-4_Rv | CGGTGTTCCTTATGTAGAGCTG |
| TmDefensin-1_Fw | AAATCGAACAAGGCCAACAC |
| TmDefencin-1_Rv | GCAAATGCAGACCCTCTTTC |
| TmDefencin-2_Fw | GGGATGCCTCATGAAGATGTAG |
| TmDefencin-2_Rv | CCAATGCAAACACATTCGTC |
| TmColoptericin-1_Fw | GGACAGAATGGTGGATGGTC |
| TmColoptericin-1_Rv | CTCCAACATTCCAGGTAGGC |
| TmColoptericin-2_Fw | GGACGGTTCTGATCTTCTTGAT |
| TmColoptericin-2_Rv | CAGCTGTTTGTTTGTTCTCGTC |
| TmAttacin-1a_Fw | GAAACGAAATGGAAGGTGGA |
| TmAttacin-1a_Rv | TGCTTCGGCAGACAATACAG |
| TmAttacin-1b_Fw | GAGCTGTGAATGCAGGACAA |
| TmAttacin-1b_Rv | CCCTCTGATGAAACCTCCAA |
| TmAttacin-2_Fw | AACTGGGATATTCGCACGTC |
| TmAttacin-2_Rv | CCCTCCGAAATGTCTGTTGT |
| TmCecropin-2_Fw | TACTAGCAGCGCCAAAACCT |
| TmCecropin-2_Rv | CTGGAACATTAGGCGGAGAA |
| TmThaumatin-likeprotein-1_Fw | CTCAAAGGACACGCAGGACT |
| TmThaumatin-like protein-1_Rv | ACTTTGAGCTTCTCGGGACA |
| TmThaumatin-like protein-2_Fw | CCGTCTGGCTAGGAGTTCTG |
| TmThaumatin-like protein-2_Rv | ACTCCTCCAGCTCCGTTACA |
| TmDorsal-X1_qPCR_Fw | AGCGTTGAGGTTTCGGTATG |
| TmDorsal-X1_qPCR_Rv | TCTTTGGTGACGCAAGACAC |
| TmDorsal-X2_qPCR_Fw | ACACCCCCGAAATCACAAAC |
| TmDorsal-X2_qPCR_Rv | TTTCAGAGCGCCAGGTTTTG |
| TmRelish_qPCR_Fw | AGCGTCAAGTTGGAGCAGAT |
| TmRelish_qPCR_Rv | GTCCGGACCTCAAGTGT |
Underline indicates T7 promoter sequences.