Table 1.
Sperm Parameters | Fresh, Pre-Freeze Semen |
Post-Thaw Semen | Total | |
---|---|---|---|---|
GSF | PSF | GSF + PSF | ||
Motility (%) | 75.78 ± 0.28 | 42.96 ± 1.63 a | 26.85 ± 1.28 b | 35.31 ± 1.65 |
MMP (%) | 87.83 ± 2.64 | 48.82 ± 1.04 a | 31.55 ± 1.55 b | 40.62 ± 1.64 |
PMI (%) | 87.43 ± 1.22 | 49.91 ± 0.84 a | 34.55 ± 1.62 b | 42.64 ± 1.51 |
NAR acrosome integrity (%) | 91.62 ± 1.07 | 53.93 ± 0.60 a | 40.85 ± 0.86 b | 47.72 ± 1.38 |
DNA fragmentation (%) | 2.97 ± 0.13 | 7.85 ± 0.45 a | 10.56 ± 0.83 b | 9.16 ± 0.50 |
LPO (nMol MDA/109spz/h) | 21.34 ± 0.68 | 27.37 ± 0.47 a | 31.55 ± 0.48 b | 29.36 ± 0.45 |
Values with different letters (a and b) are significantly differed (p < 0.05); MMP—mitochondrial membrane potential; PMI—plasma membrane integrity; NAR—normal apical ridge; LPO—lipid peroxidation; MDA—malondialdehyde.