Figure 1.

The total m⁶A content and m⁶A modification-related gene expression during osteoclast differentiation. RAW264.7 cells (A–C) and bone marrow-derived macrophages (BMMs) (D–F) were induced to differentiate into osteoclasts. (A,D) The total m⁶A content in cells was measured by quantifying m⁶A RNA methylation. (B,E) The mRNA levels of m⁶A modification-related genes were determined by real-time quantitative polymerase chain reaction (qRT-PCR). (C,F) The protein levels of m⁶A modification-related enzymes were assessed by Western blotting. Gapdh was used as an internal control. The results represent the mean ± SD (n = 3). ** P < 0.01; *** P < 0.001.