Figure 2.

Effect of Mettl3 knockdown on the m⁶A methylation levels and proliferation of osteoclast precursor cells. The efficiency of Mettl3 knockdown was determined by both Western blotting (A) and qRT-PCR (B). Mock, cells transfected with transfection reagent; Mettl3-shCtrl, cells transfected with negative control shRNA; Mettl3-shRNA, cells transfected with Mettl3 shRNA. GAPDH was used as an internal control. (C) The total m⁶A content in RAW264.7 cells and BMMs was measured by quantifying m⁶A RNA methylation. Cell growth of RAW264.7 cells (D) and BMMs (E) in Mettl3-shRNA and Mettl3-shCtrl groups was detected using a CCK8 assay after culturing for different times. The results are presented as the mean ± SD (n = 3). Significant differences relative to the Mettl3-shCtrl group. * P < 0.05; ** P < 0.01; *** P < 0.001.