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Calcium imaging of primary mouse neurons expressing NCaMP7. (a) Representative confocal images of neurons co-expressing NCaMP7 and miRFP. (b) Representative single cell recording of NCaMP7 green fluorescence responses during spontaneous neuronal activity. (c) Maximal (left) and average (right) ΔF/F for the experiment of b. (d) Time constant for the rise (left) and decay (right) of the NCaMP7 fluorescence during the calcium spikes for the experiment of b. Scale bar, 50 µm.
