Table 3.
Extracted and calculated parameters of fast kinetics Chl a fluorescence (according to Jiang et al. [63], Kalaji et al. [83]).
| Extracted parameters | Calculated parameters | ||
| FO | Minimum fluorescence, when all PSII reaction centers (RCs) are open | Fv | variable fluorescence; Fm − F0 |
| FM | Maximum fluorescence, when all PSII reaction centers are closed | Fv/Fm | maximum quantum yield of PSII; (Fm − F0)/Fm |
| F50μs, F100μs, F300μs, F2ms, F30 ms | Fluorescence intensities at 50, 100, 300 μs, 2, 30 ms, respectively | Fv/F0 | activity of the water-splitting complex on the donor side of the PSII; (Fm − F0)/F0 |
| Area | Total complementary area between fluorescence induction curve and F = Fm | ||
| OJIP parameters (calculated) | Yields or flux ratios (calculated) | ||
| VJ | Relative variable fluorescence at 2 ms (J-step); VJ = (F2ms − Fo)/(Fm − Fo) | φPo | Maximum quantum yield of primary photochemistry at t = 0; φPo = 1 − Fo/Fm = Fv/Fm |
| VI | Relative variable fluorescence at 30 ms (I-step); VI = (F30ms − Fo)/(Fm − Fo) | φEo | Quantum yield for electron transport at t = 0; φEo = (Fv/Fm)(1 − VJ) |
| Sm | Normalized total complementary area above the OJIP transient (reflecting multiple-turnover QA reduction events) or total electron carriers r RC; Sm = Area/(Fm − Fo) | ψEo | Probability (at time 0) that trapped exciton moves an electron into the electron transport chain beyond; ψEo = 1 − VJ |
| ρRo | Efficiency with which a trapped exciton can move an electron into the electron transport chain from QA‾ to the PSI and electron acceptors; ρRo = ψEoδRo = (1 − VJ)(1 − VI)/(1 − VJ) | ||
| δRo | Efficiency with which an electron can move from the reduced intersystem electron acceptors to the PSI end electron acceptors; δRo = REo/ETo = (1 − VI)/(1 − VJ) | ||
| φRo | Quantum yield for the reduction of end acceptors of PSI per photon absorbed; φRo = REo/ABS = φPoψEoδRo | ||
| Specific fluxes or activities per reaction center (RC) (calculated) | Phenomenological fluxes or activities per excited cross section (CS) (calculated) | ||
| ABS/RC | Absorption flux per RC; ABS/RC = Mo/VJ = 4(F300μs − Fo)/(Fm − Fo)/VJ | TRo/CSo | Trapped energy flux per CS at t = 0; TRo/CSo = (ABS/CSo)φPo |
| TRo/RC | Trapped energy flux per RC at t = 0; TRo/RC = Mo/VJ | ETo/CSo | Electron transport flux per CS at t = 0; ETo/CSo = (ABS/CSo)φEo |
| ETo/RC | Electron transport flux per RC at t = 0; ETo/RC = (Mo/VJ)ψEo | DIo/CSo | Dissipated energy flux per CS at t = 0; DIo/CSo = ABS CSo − TRo/CSo |
| DIo/RC | Dissipated energy flux per RC at t = 0; DIo/RC = ABS/RC − TRo/RC | ||
| Density of reaction centers (calculated) | |||
| RC/CSo | Amount of active PSII RCs per CS at t = 0; RC/CSo = φPo(ABS/CSo)(VJ/Mo) | ||