Presence of Lamin A immunopositive pyramidal neurons in CA1 and CA3. Neurons from adult (A,F) and elderly (B,G) subjects lack immunopositivity to Lamin A. Increased membrane expression of Lamin A characterizes early AD stages (C,H) and the presence of immunopositivity continues at late AD stages (D,E,I,J), 40X microphotographs, scale bar—10 µm. Quantification of immunopositivity. Significant increases of Lamin A in total intensity (K), percentage of positive cells (L), and mean intensity (M). Each point represents the image analysis of 50 cells per subject in CA1 and CA3 regions. Graphs express mean ± SD, * p < 0.05. See the text for further details of image analysis and statistics.