Table 1.
S-nitrosylated proteins identified in guard cells after flg22 treatment for 15 min, 30 min and 60 min. The tick under DiANNA indicates the predicted cysteine residue involved in disulfide bond formation.
| Protein Accession | Peptide | Protein Description | Fold Change |
p-value (FDR-adj.) | Biological Function | DiANNA |
|---|---|---|---|---|---|---|
| 15 min after flg22 treatment | ||||||
| AT2G10940.1 | NPPPGYTCSI | Lipid-transfer protein (LTP) II | 4.72 | 0.016 | Lipid binding | √ |
| AT2G10940.1 | ATCPIDTLK | LTP II | 4.67 | 0.025 | Lipid binding | √ |
| AT3G23810.1 | FDNLYGCR | S-adenosyl-L-homocysteine hydrolase 2 (SAHH2) | 3.29 | 0.024 | Carbon metabolic process | √ |
| AT3G44310.2 | IGAAICWENR | Nitrilase (NIT) 1 | 3.18 | 0.003 | Nitrogen compound metabolic process | √ |
| AT3G08560.1 | IVCENTLDAR | Vacuolar H+-ATPase (VHA) E2 | 2.94 | 0.001 | Hydrogen ion transport | √ |
| AT1G78830.1 | CLGYFYK | Curculin-like lectin protein (CLP) | 1.91 | 0.020 | Response to cytokinin, Stress response | √ |
| AT1G78850.1 | GLLGWDETCK | Mannose binding lectin (MBL) 1 | 1.90 | 0.010 | Mannose binding | √ |
| AT1G78850.1 | SPSLASCDPK | MBL 1 | 1.67 | 0.039 | Mannose binding | √ |
| AT5G54270.1 | WAMLGAFGCITPEVLQK | Chlorophyll a-b binding protein (CBP) 3 | 1.48 | 0.032 | Photosynthesis | − |
| 30 min after flg22 treatment | ||||||
| AT2G10940.1 | NPPPGYTCSI | LTP II | 2.22 | 0.005 | Lipid binding | √ |
| AT5G17220.1 | LYGQVTAACPQR | Glutathione S-transferase (GST) phi 12 | 1.53 | 0.049 | Nitrogen compound metabolism, Stress response | √ |
| AT1G26850.3 | CLIPWGANDGMYLMEVDR | S-adenosyl-L-methionine- methyltransferase (SAMMT) | 0.64 | 0.035 | Methylation | √ |
| AT5G54270.1 | WAMLGAFGCITPEVLQK | CBP 3 | 0.57 | 0.047 | Photosynthesis | − |
| AT1G78850.1 | GLLGWDETCK | MBL1 | 0.55 | 0.023 | Mannose binding | √ |
| AT1G65590.1 | VVPFEPGSCLAQ | Beta-hexosaminidase (HAD) 3 | 0.54 | 0.021 | Lipid metabolic process | √ |
| AT3G01500.1 | VCPSHVLDFQPGDAFVVR | Beta-carbonic anhydrase (CA) 1 | 0.49 | 0.047 | Carbon utilization, Stress response | √ |
| AT4G39710.1 | SGLGFCDLDVGFGDEAPR | FK506-binding protein (FKBP) 16-2 | 0.43 | 0.012 | Photosynthesis | √ |
| AT5G63800.1 | SPDAPDPVINTCNGMK | Beta-galactosidase (GALD) 6 | 0.38 | 0.035 | Carbohydrate metabolism | √ |
| AT3G52500.1 | YLCSGCDFSGLDPTLIPR | Aspartyl protease (APP) | 0.30 | 0.039 | Cellular process | √ |
| AT4G28520.3 | VVPGCAETFMDSQPMQGQQQGQPWQGR | Cruciferin (CRU) 3 | 0.15 | 0.045 | Response to abscisic acid | √ |
| AT5G44120.3 | VIPGCAETFQDSSEFQPR | CRU 1 | 0.10 | 0.027 | Response to abscisic acid | √ |
|
60 min after flg22 treatment AT3G44310.2 AT3G44310.3 |
CIWGQGDGSTIPVYDTPIGK | NIT 1 | 6.31 | 0.049 | Nitrogen compound metabolic process | √ |
| AT3G23810.1 | FDNLYGCR | SAHH2 | 3.29 | 0.024 | Cysteine and methionine metabolism | √ |
| AT4G11150.1 | IVCENTLDAR | VHA E1 | 2.55 | 0.003 | ATP synthesis | √ |
| AT2G05520.1 | QGGGGSGGSYCR | Glycine-rich protein (GRP) 3 | 2.12 | 0.028 | Stress response | √ |
| ATCG01060.1 | CESACPTDFLSVR | PSI PsaC subunit | 2.11 | 0.031 | Photosynthesis | √ |
| AT5G38410.3 | QVQCISFIAYKPPSFTEA | Ribulose bisphosphate carboxylase (Rubisco) small chain (RBCS) | 2.03 | 0.049 | Photosynthesis | √ |
| AT2G30970.1 | IAAVQTLSGTGACR | Aspartate aminotransferase (APAT)1 | 1.99 | 0.023 | Amino acid metabolic process | √ |
| AT2G21060.1 | ECSQGGGGYSGGGGGGR | GRP 2 | 1.96 | 0.030 | Stress response | √ |
| AT3G62940.3 | LKPLGLTVSEIKPDGHCLYR | Cysteine proteinase (CP) | 1.91 | 0.008 | - | √ |
| AT5G16390.1 | QLDCELVIR | Acetylcoenzyme A carboxylase (AAC) 1 | 1.71 | 0.001 | Fatty acid biosynthetic process | √ |
| ATCG00490.1 | VALEACVQAR | Rubisco Large chain (RBCL) | 1.71 | 0.019 | Photosynthesis | √ |
| AT2G20360.1 | YIQVSCLGASVSSPSR | NAD (P)-binding protein (NBP) | 1.45 | 0.018 | Electron transport, Stress response | − |
| AT5G08680.1 | CALVYGQMNEPPGAR | ATP synthase subunit (ASS) beta-3 | 1.45 | 0.046 | ATP synthesis | − |
| AT5G23890.1 | VIETDTQPSDLCTR | Unknown protein (UNK) | 1.45 | 0.007 | − | √ |
| AT3G09820.1 | AGCYASNVVIQR | Adenosine kinase (ADK) 1 | 1.42 | 0.010 | Purine metabolism, response to stimuli | √ |
| AT5G03340.1 | YTQGFSGADITEICQR | Cell division cycle (CDC) 48 | 1.30 | 0.041 | Protein transport, cell division | √ |
| AT4G13010.1 | LANAHVTATCGAR | Oxidoreductase (OR) | 1.28 | 0.046 | Oxidation and reduction | √ |
| AT1G42970.1 | TNPADEECKVYD | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) B | 0.79 | 0.031 | Glucose metabolism, Oxidation and reduction | √ |
| AT5G36700.1 | ENPGCLFIATNR | 2-phosphoglycolate phosphatase (PGP) 1 | 0.76 | 0.043 | Photorespiration | √ |
| AT3G01500.3 | YMVFACSDSR | CA 1 | 0.69 | 0.021 | Carbon utilization | − |
| AT5G14740.1 | VLAESESSAFEDQCGR | CA 2 | 0.69 | 0.007 | Carbon utilization | − |