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. 2020 Mar 3;21(5):1711. doi: 10.3390/ijms21051711

Figure 5.

Figure 5

Claudin-11 expression is significantly decreased in symptomatic Tg2576. (a,b) immunohistochemical staining of oligodendroglia-specific claudin-11 shows significant loss of claudin-11 expression in Tg2576 mice at 15 months (Ag-Tg), but not at 6 months (Yg-Tg). (c) Transcriptomic analysis using Taqman probes targeting cubilin in the ileum of Tg2576 and WT littermate controls shows nearly undetectable Cubilin gene expression in the ileum of Tg2576 mice at 6 months (Yg-Tg) compared to the WT littermate controls (Yg-WT). (d) Metabolomics analysis using mass spectrometry targeting B12 in the blood plasma of Tg2576 and WT littermate controls shows low levels of B12 in the plasma of Tg2576 mice at 15 months (Ag-Tg) compared to the age-matched WT littermate controls (Ag-WT) (p < 0.06). (a) Green: Claudin-11, Blue: DAPI nuclei. Magnification 60×. Bar indicates 25 µm. n = 3 per group. Data are expressed as mean ± SEM, as well as individual values, and are obtained from >two independent experiments at various times. * p < 0.05, ** p < 0.01, P values were calculated using Two-Way ANOVA analysis with Tukey’s multiple comparisons test (b–d).