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. 2020 Mar 9;21(5):1857. doi: 10.3390/ijms21051857

Figure 6.

Figure 6

Analysis of polyamines, mRNA abundance and yeast one-hybrid assay for OsHSFA3. (A–C) Free polyamine contents in WT and OsHSFA3-overexpressing transgenic lines before and after drought stress. Put, putrescine; Spd, spermidine; Spm, spermine. Values indicate mean ± SE of 3 biologically independent samples. Significance of data was tested with Student’s t test. * represents p < 0.05, ** represents p < 0.01. (D–G) Quantitative real-time PCR analysis of expression levels of stress-responsive genes in WT and OsHSFA3-overexpressing transgenic plants under normal and drought conditions. (H) Yeast one-hybrid assay showing that OsHSFA3 can bind to AtADC1 and OsADC in vitro on a triple-dropout medium.