Table 1.
Comparison of total lipid peak area and major lipid classes of R. qingshengii 1139 obtained by bead beating and sonication and detected by TLC-FID.
| Lipid Extraction Methods | Total Peak Area (µV/s) | Detectable Lipid Classes (mg/500 µL) by TLC-FID | Lipid % of Cell Dry Weight | |||||
|---|---|---|---|---|---|---|---|---|
| NPL | TAG | FFA | MA | PL/TDM | Total | |||
| Bead beating (unbound) | 24410 | 2.20 | 0.37 | 0.69 | - | 2.45 | 5.71 | 28.6 |
| Bead beating (bound) a | 284 | - | - | - | - | - | - | 1.2 |
| Sonication (unbound) | 11789 | 1.24 | 0.26 | 0.59 | - | 1.50 | 3.59 | 18.0 |
| Sonication (bound) a | 5098 | 0.49 | - | 0.97 | - | 0.37 | 1.83 | 9.2 |
a Lipid extracted from saponified cell debris after extraction of unbound lipids.