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Extension of the 3’-poly(a) sequence improves the acceleration effect. (a) mRNA constructs with the gradually increasing length of the 3’-poly(A) sequence; (b) kinetic curves of the translation of indicated mRNAs in a Krebs-2 system; (c) effect of the length of 3’-poly(A) on the translation acceleration rate. The lengths of minimal poly(A) sequences that can bind one or two poly(A)-binding protein (PABP) molecules are indicated by arrows.
