Table 2.
Comparison of viral tracers of anterograde trans-mulisynaptic tracing
| Strain | Genome | Dir | Cell toxicity | Days of survival | Expressing level | Operation risk | Cre/flp dependent |
|---|---|---|---|---|---|---|---|
| WT-VSV |
-ss RNA 12 kb |
A | High | 3–5 | High | Low | N |
| VSV-N(R7A) |
-ss RNA 12 kb |
A | Medium | 10–15 | High | Low | N |
| HSV H129 |
ds DNA 153 kb |
A/R | High | 3–5 | Medium | Medium | Y |
| HSV H8 |
ds DNA 153 kb |
A/R | High | 3–5 | High | Medium | Y |
Abbreviations: -ss RNA Negative-strand, single strand RNA, ds DNA Double strand DNA, Dir Direction of transsynaptic tracing, “A” Anterograde, “R” Retrograde, “N” Can’t be used as a cre/flp dependent anterograde trans-multisynaptic viral tracer, “Y” Can be used as a cre/flp dependent anterograde trans-multisynaptic viral tracer. VSV is a negative-stranded RNA virus, HSV is a double stranded DNA virus, there are many different characteristics between them, including genome, toxicity and application, etc. Researchers should choose the virus according to different experimental needs