Skip to main content
. 2020 Feb 17;16(8):1303–1323. doi: 10.7150/ijbs.38962

Figure 2.

Figure 2

Figure 2

Figure 2

Overexpression of ALDH2 confers a robust neuroprotective effect against acute stroke. (A, B) Overexpression of ALDH2 expression was achieved by lentivirus transfection and confirmed by Western blot analysis. C. Neurological deficit scores of each group. *, P < 0.05, compared to MCAO group; #P < 0.05, compared to sham group. D. Selected images of TTC-stained brain slices. The white areas are defined as the infarct regions. E. Statistical analysis of infarct volume percentages. F. Selected photographs of in situ detection of apoptotic neurons by TUNEL staining; G. Percentage of TUNEL-positive nuclei in the ischemic penumbra. Scale bar, 100μm. H. and I. Quantitative analysis of relative 4-HNE and MDA content respectively. Sham, sham-operated; MCAO, focal cerebral ischemia-reperfusion injury induced by middle cerebral artery occlusion; ALDH2-OE, the MCAO model was established one week after intracerebroventricular injection of lentivirus-ALDH2 overexpressing vectors. ALDH2-NC, the MCAO model was set up one week after intracerebroventricular injection of lentivirus of negative control vectors. Mean ± SD was used to describe the data (n = 6-8 in each group). # indicated P< 0.05.