Figure 8.

Inhibition of p110δ suppresses Akt activation and pathological angiogenesis in a mouse model of OIR. Litters of P12 mice that had been exposed to 75% oxygen for 5 days were injected intravitreally with idelalisib (Idel) (final vitreal concentration of ∼10 μmol/L) or its vehicle (V) (0.1% DMSO) and then stayed in room air for 5 days. A: At P17, whole-mount retinas were stained with IB4. B and C: Analysis of neovascularization areas (tufts) from the IB4-stained retinas (n = 6) and avascular area (n = 6). D and E: Total lysates from retinas (one retina/lane) were subjected to Western blot analysis using the indicated antibodies. Data are mean ± SD of three independent experiments (folds). *P < 0.05, ****P < 0.0001 between two compared groups by unpaired t test. N, retinas from P17 mice without experiencing 75% high oxygen.