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Primer sequences
| Gene names | Primer sequences (5'-3') |
|---|---|
| ACSL1 | |
| F | CCATGAGCTGTTCCGGTATTT |
| R | CCGAAGCCCATAAGCGTGTT |
| SREBP1 | |
| F | CGGAACCATCTTGGCAACAGT |
| R | CGCTTCTCAATGGCGTTGT |
| SCD | |
| F | TTCCTACCTGCAAGTTCTACACC |
| R | CCGAGCTTTGTAAGAGCGGT |
| ACC | |
| F | ATGTCTGGCTTGCACCTAGTA |
| R | CCCCAAAGCGAGTAACAAATTCT |
| PPARγ | |
| F | ACCAAAGTGCAATCAAAGTGGA |
| R | ATGAGGGAGTTGGAAGGCTCT |
| FAS | |
| F | AAGGACCTGTCTAGGTTTGATGC |
| R | TGGCTTCATAGGTGACTTCCA |
| GAPDH | |
| F | GGAGCGAGATCCCTCCAAAAT |
| R | GGCTGTTGTCATACTTCTCATGG |
F, forward; R, reverse.
