. Author manuscript; available in PMC: 2020 Dec 12.

Published in final edited form as: Biochem J. 2019 Dec 12;476(23):3661–3685. doi: 10.1042/BCJ20190532

Table 3.

Effect of incorporation of CYP2E1 into HLM-2 on the kinetic parameters of oxidation of p-Nitrophenol and Chlorzoxazone^*

Molar ratio of added CYP2E1 to P450 present in HLM	CYP2E1 content, nmol/mg protein	p-Nitrophenol			Chlorzoxazone
Molar ratio of added CYP2E1 to P450 present in HLM	CYP2E1 content, nmol/mg protein	S₅₀, μM^a	N^a	V_max, min⁻¹	K_M, μM	V_max, min⁻¹
0	0.028	39 ± 17	1.3 ±0.2	6.3 ± 3.6	54 ± 16	12 ± 3
0.25	0.13	43 ± 7	1.3 ±0.4	12 ±6	79 ±29	42 ± 11
0.5	0.23	37 ± 11	1.6 ±0.3	29 ± 13	54 ± 4.4	42 ±4
0.75	0.32	81 ± 11	1.7 ±0.2	26 ±8	84 ± 13	105 ±25
1	0.42	81 ± 5	1.2 ±0.3	33 ± 12	65 ±20	78 ±8
1.75	0.72	48 ±2	1.5 ±0.4	27 ±9	87 ± 18	54 ± 11

The estimates given in the table correspond to the averages of 2–4 individual measurements. The“±” values are the confidence intervals calculated for p=0.05.

S₅₀ are N are the concentration of half-saturation with substrate and the Hill coefficient obtained from the fitting of the titration curves with the Hill equation.