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Overexpression and silencing of USP5 by using lentivirus. (a, b) RA-FLS were infected with lentivirus containing empty plasmid (control group) or USP5 expression plasmid (USP5 group) for 48 hours; the efficiency was confirmed in both mRNA (a) and protein (b) levels. RA-FLS were infected with lentivirus containing negative control shRNA plasmid (sh-NC group) or USP5 shRNA plasmid (sh-USP5) for 48 hours; the efficiency was confirmed in both mRNA (c) and protein (d) levels. ∗∗∗p < 0.001.
