KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse anti-α-tubulin, clone DM1 | Sigma | Cat# T6199; RRID:AB_477583 |
| Human anti-centromere | Antibodies Incorporated | Cat# 15–234; RRID:AB_2687472 |
| Rabbit anti-γ-tubulin | Sigma | Cat# T3559; RRID:AB_477575 |
| Mouse anti-centrin, clone 20H5 | Millipore | Cat# 04–1624; RRID:AB_10563501 |
| Rabbit anti-TPX2 | Sigma | Cat# HPA005487; RRID:AB_1858223 |
| Mouse anti-MAD1, clone BB3–8 | Millipore | Cat# MABE867; RRID:AB_831526 |
| Anti-β-actin-HRP | Santa Cruz | Cat# sc-47778; RRID:AB_2714189 |
| Anti-c-MYC, clone Y69 | Abcam | Cat# ab32072; RRID:AB_731658 |
| Anti-cleaved PARP | Cell Signaling Technology | Cat# 9542; RRID:AB_2160739 |
| Rabbit anti-Cyclin B1 | Abcam | Cat# ab2949 |
| Rabbit anti-Cyclin A, clone C-19 | Santa Cruz Biotechnology | Cat# sc-596; RRID:AB_631330 |
| Anti-mouse conjugated to Alexa Fluor 488 | Thermo Fisher | Cat# A11029; RRID:AB_138404 |
| Anti-human conjugated to Alexa Fluor 594 | Thermo Fisher | Cat# A11014; RRID:AB_2534081 |
| Anti-rabbit conjugated to Alexa Fluor 647 | Abcam | Cat# ab150079; RRID:AB_2722623 |
| Biological Samples | ||
| Patient-derived xenogafts (PDX) | DeRose et al., 2011 | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Nocodazole | Sigma | Cat# M1404 |
| Purvalanol A | Sigma | Cat# P4484 |
| Propidium iodide | Sigma | Cat# P4170 |
| RNase A | Sigma | Cat# R6513 |
| Critical Commercial Assays | ||
| PrestoBlue Cell Viability Reagent | Thermo Fisher | Cat# A13261 |
| Guava ViaCount Viability Assay | Millipore | Cat# 4000–0040 |
| Trypan Blue Stain | Thermo Fisher | Cat# T10282 |
| ChIP DNA purification kit | Active Motif | Cat# 58002 |
| Power Up Sybr Master Mix | Thermo Fisher | Cat# A25741 |
| Deposited Data | ||
| RNA sequencing of Eμ-tTA/TRE-MYC lymphoma cells and MMTV-rtTA/TetO-MYC tumors | This paper | GEO: GSE130922 |
| Gene expression values of LAP-tTA × TetO-MYC (LT2-MYC) liver tumors | Anderton et al., 2017 | GEO: GSE73295 |
| The Cancer Genome Atlas | Cancer Genome Atlas Network, 2012 | GEO: GSE62944 |
| Experimental Models: Cell Lines | ||
| RPE-NEO | Goga et al., 2007 | N/A |
| RPE-MYC | Goga et al., 2007 | N/A |
| MDA-MB-231 | ATCC | HTB-26 |
| HCC1143 | ATCC | CRL-2321 |
| BT549 | ATCC | HTB-122 |
| HCC3153 | Neve et al., 2006 | N/A |
| HCC1428 | ATCC | CRL-2327 |
| T47D | ATCC | HTB-133 |
| LY2 | Neve et al., 2006 | N/A |
| ZR75B | ATCC | CRL-1500 |
| HMEC MYC ER | Horiuchi et al., 2012 | N/A |
| eμ-tTX/TRE-MYC cells | Goga et al., 2007 | N/A |
| MTB-TOM cells | This study | N/A |
| 293T | ATCC | CRL-3216 |
| Rat1a | Goga et al., 2007 | N/A |
| Rat1a myc−/−MYC | Schwinkendorf and Gallant, 2009 | N/A |
| Rat1a myc−/− MYCΔMBII | Schwinkendorf and Gallant, 2009 | N/A |
| RPE-MYC shTPX2 CDS1 | This study | N/A |
| RPE-MYC shTPX2 CDS2 | This study | N/A |
| RPE-MYC shTPX2 CDS3 | This study | N/A |
| RPE-MYC shGFP | This study | N/A |
| RPE-MYC shResTPX2-mEm | This study | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: MMTV-rtTA/TetO-MYC | D’Cruz et al., 2001 | N/A |
| Mouse: NOD/MrkBomTac-Prkdcscid | Taconic Biosciences | N/A |
| Oligonucleotides | ||
| shRNA sequences see Table S3 | This study | N/A |
| Primer for TPX2 shRNA resistant see Table S3 | This study | N/A |
| Primer for qPCR see Table S3 | This study | N/A |
| Primer for ChIP PCR see Table S3 | This study | N/A |
| Recombinant DNA | ||
| pCDII-EF-MCS mKO2-hCdt1(30/120) | Sakaue-Sawano et al., 2008 | GenBank accession# AB370332 |
| pCDII-EF-MCS mAG-hGeminin(1/110) | Sakaue-Sawano et al., 2008 | GenBank accession# AB370333 |
| pLenti6/V5-DEST H2B-mCherry | Pemble et al., 2017 | N/A |
| Tet-PLKO-puro | Wiederschain et al., 2009 | Addgene Plasmid #21915 |
| pHR-mEmerald | Hu and Mullins, 2019 | N/A |
| Software and Algorithms | ||
| Fiji | Schindelin et al., 2012 | https://fiji.sc/ |
| STAR aligner | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| edgeR package | Robinson et al., 2010 | https://doi.org/10.18129/B9.bioc.edgeR |
| DESeq2 package | Love et al., 2014 | https://doi.org/10.18129/B9.bioc.DESeq2 |
| limma R package | Ritchie et al., 2015 | https://doi.org/10.18129/B9.bioc.limma |