(A) Schematic timeline of the fibrotic and control mice with a double dose of CCl4 (0.4 ml/kg) (without K.P infection). (B, C) Serum IL-6 and IFN-γ, and relative mRNA expressions of Il-6 and Ifng, and their downstream target genes in the liver were determined. (D) Western blot analyses of IL-6 and IFN-γ downstream signaling pathways. (E) Kupffer cells were isolated from control and fibrotic livers and stimulated with LPS. The IL-6 mRNA and protein (in the supernatant) were measured. Student’s t-test was used for statistical evaluation (*P<0.05, **P<0.01, ***P<0.001, n= 3-5 in panel B-E).