Fig. 6.

MEK5 depletion impairs non-homologous end joining. a intact or b HindIII-digested pEGFP-N1 vector was transiently transfected in PC3 cells expressing shControl or shMEK5. shControl cells were also treated or not with NU7441. Twenty-four hours post-transfection, cells were fixed, stained with DAPI, and EGFP-positive cells were quantitated by fluorescence as percent EGFP-positive cells/total (DAPI) number cells. Mean ± S.D. (n = 3). P-value was calculated by Student’s t-test. UT: uncut plasmid; HindIII: restriction enzyme-digested plasmid.