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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Oncogene. 2020 Jan 27;39(12):2583–2596. doi: 10.1038/s41388-020-1167-x

Figure 3: PACS-1 localizes to chromatin and regulates the stability of HDAC2 and HDAC3 proteins.

Figure 3:

A) iPOND followed by analysis with mass spectrometry reveals decreased HDAC2 in siPACS-1 cells. B) Level of class I HDACs in control and PACS-1 downregulated cells. C) iPOND assay reveals PACS-1 localization to the sites of DNA replication and it is important for HDAC2 and HDAC3 stability in the replication forks and to maintain chromatin regulation. D) Acetylation status of the HDAC2 and HDAC3 specific histone modification in control and PACS-1 down regulated cells. E) Depletion of PACS-1 leads to decreased HP1, marker for heterochromatin. F) MNase assay results of nuclei isolated from control vs PACS-1 knockdown cells. **p < 0.01.