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. 2020 Feb 5;37(4):800–814. doi: 10.1007/s12640-020-00166-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — The effect of chronic constriction injury (CCI) on the dopamine D1 and D2 receptor mRNA and protein levels in the mesostriatal system. a, b An in situ hybridization analysis of D2 receptor gene expression at 7 days after CCI. Panel a shows representative autoradiograms, where optical densities are color-coded according to the scale on the left (PSL, photostimulated luminescence units). The bars in panel b represent the mean (± S.E.M.) optical densities in the indicated brain regions of the CCI group (n = 6), ipsilateral and contralateral to the injured nerve. The data are expressed as a percentage of the corresponding values in the naive control group (n = 5). The control is indicated by the horizontal line at 100%, and the vertical error bar on the right-hand side of this line represents the mean S.E.M. in the control group. p and F values above the bars are the results of three-way ANOVAs (treatment × side × region of interest [ROI]). All other results of these ANOVAs, not shown in the figure, were nonsignificant, except for the ROI factor (which reflected differences in the level of the D2 receptor gene expression between brain regions). c, d qRT-PCR measurements of the D1 and D2 receptor mRNA levels in the nucleus accumbens at 14 days after CCI. Data represent the mean (± S.E.M.) transcript abundance standardized against HPRT1 transcript and expressed as percentage of control (see description of panel b); n = 8 to 10. p and F values above the bars are the results of two-way ANOVAs (treatment × side). The side factor and treatment × side interaction were nonsignificant. e, f Western blot measurements of the D1 and D2 receptor protein levels in the nucleus accumbens at 14 days after CCI. Data represent the mean (± S.E.M.) expressed as percentage of control; n = 4 to 5. p and F values above the bars are the results of two-way ANOVAs (treatment × side). The side factor and treatment × side interaction were nonsignificant in both cases. For the D1 receptor, the treatment factor was also nonsignificant. All ANOVAs were performed on raw data. dStr, dorsal striatum; NAc, nucleus accumbens; SNc, substantia nigra pars compacta; VTA, ventral tegmental area