Table 1.

Comparison of N-glycosylation sites between spike glycoproteins of 2019-nCoV and SARS-CoV strains

N-glycosylation
2019-nCoV						SARS-CoV
Position (NetNGlyc 1.0) agreement result	Position (N-GlyDE)	Sites	Potential	Jury agreement	N-Glyc Result	Position (NetNGlyc 1.0) agreement result	Position (N-GlyDE)	Sites	Potential	Jury agreement	N-Glyc Result
–	–	–	–	–	–	29	29	NYTQ	0.7751	(9/9)	+++
61	61	NVTW	0.7820	(9/9)	+++	65	65	NVTG	0.8091	(9/9)	+++
234	234	NITR	0.7613	(9/9)	+++	227	227	NITN	0.7518	(9/9)	+++
74	74	NGTK	0.7192	(9/9)	++	119	119	NSTN	0.7039	(9/9)	++
282	282	NGTI	0.7378	(9/9)	++	269	269	NGTI	0.6910	(9/9)	++
616	616	NCTE	0.7163	(9/9)	++	318	318	NITN	0.6413	(9/9)	++
717	717	NFTI	0.6426	(9/9)	++	602	602	NCTD	0.6916	(9/9)	++
1194	1194	NESL	0.6791	(9/9)	++	783	783	NFSQ	0.6260	(9/9)	++
17	17	NLTT	0.6606	(8/9)	+	1176	1176	NESL	0.6794	(9/9)	++
122	122	NATN	0.6781	(8/9)	+	73	73	NHTF	0.5303	(4/9)	+
149	149	NKSW	0.6318	(7/9)	+	109	109	NKSQ	0.6080	(7/9)	+
165	165	NCTF	0.6220	(8/9)	+	158	158	NCTF	0.5808	(7/9)	+
331	331	NITN	0.5970	(7/9)	+	330	330	NATK	0.6063	(8/9)	+
343	343	NATR	0.5671	(8/9)	+	357	357	NSTS	0.6836	(8/9)	+
603	603	NTSN	0.5783	(6/9)	+	589	589	NASS	0.5777	(6/9)	+
801	801	NFSQ	0.6146	(8/9)	+	699	699	NFSI	0.5356	(7/9)	+
1098	1098	NGTH	0.5496	(5/9)	+	1080	1080	NGTS	0.5806	(7/9)	+
1134	1134	NNTV	0.5800	(6/9)	+	1116	1116	NNTV	0.5107	(5/9)	+

The table shows a comparison of predicted N-glycosylation sites in Spike glycoprotein of Wuhan-Hu-1–2019 novel coronavirus (2019-nCoV) and SARS coronavirus-2003 (SARS-CoV) strains. N-glycosylation potential (0.5) was taken as cutoff. The N-glycosylation sites were determined by NetNGlyc 1.0 [20] and validated by N-GlyDE server [21]

*Italics indicates the differences between N-glycosylation sites between the two Spike glycoproteins