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. 2020 Jan 27;31(1):38–44. doi: 10.1007/s13337-019-00566-x

Fig. 2.

Fig. 2

a Detection of PYMoV through optimized RPA assay using CTAB isolated DNA as template. Lane M: 100 bp DNA ladder; Lane 1: DNA from infected plant; Lane 2: DNA from healthy plant; Lane 3: Water control. b Detection of PYMoV through optimized RPA assay using crude DNA extract. Lane 1: crude DNA extract from infected plant; Lane 2: 1:10 diluted DNA crude extract from infected plant; Lane 3: crude DNA extract from healthy plant; Lane 4: 1:10 diluted crude DNA extract from healthy plant; Lane 5: Water control. c Detection of PYMoV through reverse transcription (RT)-RPA assay using cDNA as template. Lane 1: cDNA from infected plant; Lane 2: cDNA from healthy plant; Lane 3: Water control