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. 2020 Feb 28;20(5):1317. doi: 10.3390/s20051317

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

(A) Microwell-patterned microfluidic analysis chip. Reprinted with permission from [121]. Copyright (2018), Royal Society of Chemistry. (B) Schematic illustration of the assembled chip of the MERCA system for nucleic acid detection. The microfluidic chamber is functionalized with probes to capture target miRNAs; (bottom) design of the MERCA chip. The three-layer PDMS/glass chip integrates seven units working parallelly, consisting of an assay chamber with a three-valve pump. Reprinted with permission from [123]. Copyright (2019), Science Direct. (C) Microbiochip integrating a unit for nucleic acid extraction, preconcentration and sensing. Reprinted with permission from [124]. Copyright (2015), Elsevier. (D) Principle of detection and chemical-free lysis of exosomes for an integrated microfluidic platform for miRNA analysis. Licensed under CC BY-NC 4.0 International License.