Figure 1.

Representation of the robotic workstation worktable and plates layout for the measurement of EST2 residual activity. (a) In the worktable column 1 the solvent reservoir carriers, in column 2 disposable tip carriers (10, 200, 1000 μL), in column 3 the stock solution and reagent plate in the temperature controller, in column 4 the incubation plate on the heated shaker, and in column 5 the plates for the enzymatic residual activity assays, were placed. (b) Plate placed in column 3 of (a), prepared by the operator manually, containing the enzyme (EST2) and the NBS treated OP inhibitors (I.1, I.2, … I.n) and a blank reference (R). (c) Plate in column 4 of (a), containing the incubation mixtures (enzyme-inhibitors) prepared by the robotic liquid handler. (d) Plate layout in column 5 for the assays of enzymatic residual activity, samples are divided in three groups for the assay of each mixture. The arrows indicated the direction of measurements for each group of assays.