Figure 1. Iron accumulation in the tissues of BKO mice.

Perl’s Prussian blue staining of representative paraffin-embedded tissue sections from (A–D) the liver, (E–H) spleen and (I–L) duodenum of male WT and BKO mice aged 6–7 weeks and 16–20 weeks. The blue stain represents iron accumulation. The arrows indicate iron accumulation in brush border of enterocytes (Nikon ECLIPSE 80i light microscope; magnification ×400).