Table 4. Mononuclear cells and hematopoietic cells score in the liver of male wild type and thalassemic mice at the age of 6–7 weeks and 16–20 weeks.

	WT		BKO		P values (2-way ANOVA)
	6–7 weeks	16–20 weeks	6–7 weeks	16–20 weeks	Age	Phenotype	Age × phenotype
Mononuclear cells and hematopoietic cells score	0.12 ± 0.02	0.16 ± 0.14	1.86 ± 0.08a	2.92 ± 0.15b,c	0.0001	<0.0001	0.0003

Notes:

^aP-value < 0.05 compared with WT aged 6–7 weeks.

^bP-value < 0.05 compared with WT aged 16–20 weeks.

^cP-value < 0.05 compared with BKO aged 6–7 weeks.

Data are expressed as mean ± SEM (n = 5/group). Statistical analysis was performed by 2-way ANOVA with Bonferroni post-hoc test.