Table 2.
Voltage dependence of activation and fast inactivation of NlNav2–1 and its recombinants.
| Na+ channel type | Activation | Inactivation | n | ||
|---|---|---|---|---|---|
| V1/2(mV) | k(mV) | V1/2(mV) | k(mV) | ||
| NlNav2–1 | 27.8 ± 0.9 | 5.6 ± 0.2 | 50.6 ± 1.0 | 5.3 ± 0.1 | 22 |
| S772A | 26.2 ± 0.7 | 6.0 ± 0.3 | 48.6 ± 0.5 | 5.3 ± 0.1 | 17 |
| Y774A | 28.1 ± 0.6 | 5.6 ± 0.2 | 49.1 ± 0.3 | 5.1 ± 0.1 | 30 |
| Y775A | 27.3 ± 0.9 | 5.4 ± 0.4 | 47.8 ± 0.8 | 5.1 ± 0.1 | 9 |
The voltage dependence of activation and fast inactivation were fitted with two-state Boltzmann equation to determine V1/2, the voltage for half-maximal conductance or inactivation, and k, the slope factor for conductance or inactivation. Values in the table represent the mean ± SEM, and n is the number of oocytes used. Voltage dependence of activation and fast inactivation have no significant different between NlNav2–1 and its recombinants, which determined by Student’s t-test analysis (p>0.05).