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. 2020 Mar 17;13:2319–2331. doi: 10.2147/OTT.S236860

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© 2020 Xiu et al.

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The inhibitor of miR-139-5p mitigated the effect of si-TUG1 on radiosensitivity, cell apoptosis, and survival fraction under 4 Gy radiation treatment. PC-3 and DU145 cells treated with irradiation were transfected with si-NC, si-TUG1#1, si-TUG1#1+ inhibitor NC or si-TUG1#1+ miR-139-5p inhibitor. (A and B) The expression of miR-139-5p was detected by qRT-PCR. (C and D) MTT assay detected the viability of PC-3 and DU145 cells. (E and F) Flow cytometry determined cell apoptosis of PC-3 and DU145 cells. (G and H) The expression level of apoptosis-related proteins (Bcl-2, Bax and cleaved-casp-3) was examined in PC-3 and DU145 cells. (I and J) The clonogenic survival curve was proven in PC-3 and DU145 cells, which were transfected with si-NC, si-TUG1#1, si-TUG1#1+ inhibitor NC or si-TUG1#1+ miR-139-5p inhibitor and subjected to 0, 2, 4, 6 and 8 Gy of irradiation. *P < 0.05.