Table 1.

Comparison of Predicted Active ERK Contents Obtained Using the Sensor Array with the Calibration Regression Model, Western Blot, and Immunoprecipitation–Kinase Assay Protocols^a

		pp-ERK (ng μg−1 Lysate)
		sensorsb		Western blot			kinase assay
cells	treatment	mean (n = 4)	±SD	mean (n = 2)	±SD	p-value (two-tailed)c p ≤ 0.05	value (n = 1)	±SEd
MDA-MB-231	M-n	0.374	0.029	0.450	0.125	0.264	0.352	0.107
	M-U	0.027	0.005	0.035	0.011	0.248	0.034	0.017
	M-E	0.898	0.031	0.656	0.071	0.003	0.643	0.103
	M-E/U	0.007	0.016	0.062	0.037	0.051	0.010	0.017
	M-J	0.067	0.013	0.110	0.055	0.168	0.073	0.012
	M-A	0.351	0.032	0.407	0.008	0.082	0.383	0.086
	M-A/J	0.412	0.014	0.402	0.005	0.422	0.421	0.057
A549	A-n	0.175	0.002	0.243	0.082	0.128	0.161	0.016
	A-U	0.155	0.004	0.202	0.041	0.059	0.141	0.051
	A-E	0.508	0.011	0.504	0.102	0.933	0.480	0.135
	A-E/U	0.211	0.009	0.302	0.086	0.074	0.236	0.225
	A-J	0.149	0.005	0.053	0.094	0.079	0.162	0.113
	A-A	0.495	0.011	0.244	0.087	0.003	0.504	0.431
	A-A/J	0.32	0.005	0.213	0.106	0.081	0.338	0.059

^aRecombinant ERK1 calibration curves were employed to generate the data in this table. Non-treated cells in serum-free media (n), inhibition of ERK phosphorylation with U0126 (U), induction of ERK phosphorylation with EGF (E), cells treated with U0126 then induced with EGF (E/U), inhibition of JNK pathway with JNK-IN-8 (J), induction of JNK phosphorylation with anisomycin (A), and cells treated with JNK-IN-8 then induced with anisomycin (A/J).

^bCell lysates were used at a concentration of 7 μg/well.

^cUnpaired t-test and p-value calculations for the sensors and the Western blot means were done using GraphPad Prism 7 software.

^dStandard error of regression slope.