Figure 2.

MESMER does not interfere with Fura-2–based Mn quantification. Various concentrations of Mn were added to empty wells (no cells) in PBS (without Ca²⁺ and Mg) in the presence of Fura-2 (0.5 μm), Fura-2 with 3 μm MESM, or Fura-2 with an equivalent DMSO vehicle. The plates were read at 360/535 nm excitation/emission as described. n = 3 of the individual replicates performed on different days is shown, with four technical replicate wells for each condition each day. Error bars show standard deviation of the three independent replicate averages. As expected, a two-way ANOVA yielded a significant main effect for Mn concentration (p < 0.0001), but no main effect of MESM or interaction. Sidak's multiple comparisons test yielded no significant differences in Fura-2% fluorescence between groups at each Mn concentration (p > 0.05).