Figure 4.

MESMER assay yields comparable readouts as current CFMEA method standard. Murine striatal neuron lineage STHdh Q7 cells (A) or HEK cells (B) were exposed to 0, 50, 100, or 200 μm Mn for 2 h at 37 °C. Q7 cells (n = 5) were exposed to Mn in HBSS and HEK cells (n = 4) in DMEM, respectively. The cells were then washed in PBS (lacking Ca²⁺ and Mg²⁺) and then exposed to 3 μm MESM (MESMER), a DMSO vehicle equivalent (vehicle), or 0.1% Triton in PBS (CFMEA). All solutions contained 0.5 μm Fura-2. After 15 min at 37 °C, the plates were read at 360/535 nm (see Fig. 3). Error bars shown represent standard deviation. Statistical significance was determined by two-way ANOVA and is denoted by the following: n.s., not significant; *, p < 0.05; ***, p < 0.001; ****, p < 0.0001.