Figure 2.

A, Growth of the WT strain and gdt1Δpmr1Δ in MD-U supplemented or not with MnCl₂. The gdt1Δpmr1Δ strain expressed GDT1, TMEM165, or its different truncated versions under the control of the constitutive TPI1 promoter. ∅, gdt1Δpmr1Δ with the empty pRS416 vector. The cells were treated as described in Fig. 1B. B, cellular Mn²⁺ content of the WT and gdt1Δpmr1Δ expressing GDT1, TMEM165, or its different truncated versions under the control of the constitutive TPI1 promoter. The strains were grown in MD-U medium to an A₆₀₀ of 3, and the cellular Mn²⁺ content was measured by ICP-AES. The data are shown as means ± S.D. (n = 4). *, p < 0.05; ***, p < 0.001 (one-way analysis of variance with Bonferroni post hoc test). ns, not significant.