Figure 3.

Two conformational states of p-DUBA observed by NMR spectroscopy. A, the overlaid ¹⁵N TROSY spectra of p-WT DUBA, p-R272E/K273E mutant, np-WT DUBA, np-R272E/K273E mutant, and S177E mutant. B, structure of DUBA (PDB code 3TMP) with residues showing two conformers in p-WT DUBA highlighted by stick-and-ball representation. C, ¹⁵N ZZ-exchange spectra of p-WT DUBA at three mixing times, showing the cross-peaks from two conformers of Ala-190. The sample temperature was 300 K for all TROSY experiments and 303 K for the ZZ-exchange experiment. The protein concentrations were ∼500 μm for all samples. C-term, C terminus; N-term, N terminus.