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. 2020 Feb 11;295(12):3851–3864. doi: 10.1074/jbc.RA119.011692

Figure 3.

Figure 3.

Effects of dksA1 gene mutation on production of QS signal molecules. A, levels of 3-oxo-C12-HSL (3-oxo-C12-HSL) were measured by using an E. coli reporter strain harboring a pKDT17 plasmid. Concentrations of 3-oxo-C12-HSL are represented as β-gal activity. Purified 3-oxo-C12-HSL (100 μg/ml) was used as the positive control. *, p < 0.05 versus β-gal activity by PAO1 culture supernatant. B, levels of N-butyryl homoserine lactone (C4-HSL) was monitored by measuring the violacein production from the biosensor strain C. violaceum CV026. Violacein production was monitored by measuring absorbance at 590 nm and normalized to the OD600 values of CV026. Purified C4-HSL (100 μg/ml) was used as a positive control. *, p < 0.05 versus values by PAO1 culture supernatant. C, PQS detection by TLC. Purified PQS (10 mm, 5 μl) was used as a positive control. Samples (10 μl) were loaded on TLC plates. White arrows, spots for PQS. Error bars, S.D.