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. 2020 Feb 11;295(12):3851–3864. doi: 10.1074/jbc.RA119.011692

Figure 4.

Figure 4.

Anerobic growth of ΔdksA1, ΔdksA2, and ΔdksA1ΔdksA2 mutants. A, growth curves of PAO1, ΔdksA1, ΔdksA2 and ΔdksA1ΔdksA2 strains in LB medium supplemented with 25 mm NO3. Aliquots of bacterial cultures (n = 3) were withdrawn every 2 h to measure OD600 values. Bacterial strains (indicated to the right) were grown anaerobically at 37 °C. B, growth curves of PAO1, ΔdksA1, ΔdksA2, and ΔdksA1ΔdksA2 strains in LB medium supplemented with 5 mm NO2. C, defective anaerobic growth of ΔdksA1 and ΔdksA1ΔdksA2 was further verified in cfu enumeration assay. Bacterial cultures after 24 h of anaerobic growth in LB supplemented with 25 mm NO3 were serially diluted, and 10 μl of each diluent was spot-inoculated onto an LB agar plate. The plates were incubated in anaerobic conditions at 37 °C for 24 h. D, anaerobic growth was not active in plain LB medium without either alternative electron acceptor. Error bars, S.D.