Table 1.
Strains and plasmids used in this study
| Bacterial strains and plasmids | Genotype or description | Reference/source |
|---|---|---|
| E. coli strains | ||
| DH5αλpir | fhuA2 lac(del)U169 phoA glnV44 Φ80′ lacZ(del)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 λpir | |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Kmr λpir | |
| P. aeruginosa strains | ||
| PAO1 | WT, laboratory strain of P. aeruginosa | This study |
| PAO1ΔdksA1 | WT with dksA1 deletion | This study |
| PAO1ΔdksA2 | WT with dksA2 deletion | This study |
| PAO1ΔdksA1dksA2 | WT with dksA1 and dksA2 double deletion | This study |
| ΔlasRrhlR | WT with lasR and rhlR double deletion | |
| Other strain | ||
| C. violaceum CV026 | Biosensor for C4-HSL | |
| Plasmids | ||
| pCVD442 | Ampr suicide vector containing sacB for screening recombinant | Ref. 10 |
| pKDT17 | Ampr pTS400 plasmid containing lasR and lasB transcriptionally fused with lacZ | Ref. 61 |
| pCVD442d1 | pCVD442-containing overlapped left- and right-flanking region of dksA1 | This study |
| pCVD442d2 | pCVD442-containing overlapped left- and right-flanking region of dksA2 | This study |
| puc18-mini-Tn7t-Gm-lacZ | Ampr, Gmr, site-specific chromosomal insertion plasmid harboring promoterless lacZ | Ref. 57 |
| pTNS2 | Helper plasmid containing site-specific recombinase for chromosomal insertion | Ref. 57 |
| pD1Tn7t-comp | Complementation plasmid contacting dksA1 fragment in puc18-mini-Tn7t-Gm-lacZ | This study |