Table 2.
Sequences of primers used in this study
| Primers | Sequence (5′–3′) | Description |
|---|---|---|
| dksA1#1 | ACA TGC ATG CAG GAA ATG CCT GTT GCA CGG | dksA1 left-flanking forward primer containing SphI restriction enzyme site |
| dksA1#2 | GCC GAG TTG CTT GAA TGG CCG CCT CTC ACT TT | dksA1 left-flanking reverse primer containing overlapping site to dksA1#3 |
| dksA1#3 | AGG CGG CCA TTC AAG CAA CTC GGC TCC TGA | dksA1 right-flanking forward primer containing overlapping site to dksA1#2 |
| dksA1#4 | TAA TGA GCT CCG GCG CTG GAT GAC GAA AT | dksA1 right-flanking reverse primer containing SacI restriction enzyme site |
| dksA2#1 | AAC ATG CAT GCA TCC TCA CCG AGG GTT TCG CCA | dksA2 left-flanking forward primer containing SphI restriction enzyme site |
| dksA2#2 | TTG GTT CAC GGT TGG TAA CCT CTT GAA CCT GGG AAA | dksA2 left-flanking reverse primer containing overlapping site to dksA2#3 |
| dksA2#3 | TGG TAA CCT CTT GAA CCT GGG AAA TTT AAA ACG TTA | dksA2 right-flanking forward primer containing overlapping site to dksA2#2 |
| dksA2#4 | TAA TGA GCT CTG GAG ACC GGC TTC ATC TCG AAT | dksA2 right-flanking reverse primer containing SacI restriction enzyme site |
| dksA1_compF | ATG GTA CCG TGG ATC ACA TCG TGG C | dksA1 complementation primer containing 300 bp upstream of dksA1 gene |
| dksA1_compR | ATA CTA GTA CCA GGG AAC CGA AGT GCA | dksA1 complementation primer containing 150 bp downstream of dksA1 gene |
| Mini-Tn7t_seqF | GCT TTT GAA GCT AAT TCG ATC A | Forward sequencing primer covering multiple cloning site of puc18-mini-Tn7t-Gm-lacZ |
| Mini-Tn7t_seqR | TCG GGA TCG CTA GTT AGT TA | Reverse sequencing primer covering multiple cloning site of puc18-mini-Tn7t-Gm-lacZ |