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. 2020 Feb 10;295(12):3837–3850. doi: 10.1074/jbc.RA119.011368

Figure 6.

Figure 6.

SNX17 endosome size increases in the absence of EHD1. A, CRISPR/Cas9 gene-edited NIH3T3 cells expressing EHD1-GFP were immunostained with anti-SNX17 antibody and imaged. B, CRISPR/Cas9 gene-edited NIH3T3 cells lacking EHD1 (EHD1-KO) were fixed, immunostained with anti-SNX17 antibody, and imaged. Insets are included to highlight the endosomal size difference. C, immunoblot demonstrating loss of EHD1 in NIH3T3 EHD1 knockout cells (top panel) and expression of EHD1-GFP (molecular weight, ∼87 kDa) in NIH3T3 EHD1-GFP cells (bottom panel). D, 3D surface rendering was carried out to encompass SNX17 voxels, and their total surface area was quantified. A grouped frequency distribution bar graph and curve (inset) for SNX17 endosome size (square micrometers) are plotted to compare endosome size distribution in EHD1-GFP (black) and EHD1-KO (red) cells. E, the relative frequency of SNX17 endosome size (of 100%) was quantified and plotted to compare the relative frequency of endosome size distribution in EHD1-GFP (black) and EHD1-KO (red) cells. Data shown are representative of six independent experiments.